Development of a rapid loop-mediated isothermal amplification assay for the detection of Dothistroma septosporum
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Date
2021-03-19
Open Access Location
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI (Basel, Switzerland)
Rights
(c) 2021 The Author/s
CC BY 4.0
CC BY 4.0
Abstract
A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.
Description
Keywords
DNA-based diagnostics, LAMP, Dothistroma needle blight
Citation
Myrholm CL, Tomm BD, Heinzelmann R, Feau N, Hamelin RC, McDougal R, Winkworth RC, Ramsfield TD. (2021). Development of a rapid loop-mediated isothermal amplification assay for the detection of Dothistroma septosporum. Forests. 12. 3.