Development of a rapid loop-mediated isothermal amplification assay for the detection of Dothistroma septosporum

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dc.citation.issue3
dc.citation.volume12
dc.contributor.authorMyrholm CL
dc.contributor.authorTomm BD
dc.contributor.authorHeinzelmann R
dc.contributor.authorFeau N
dc.contributor.authorHamelin RC
dc.contributor.authorMcDougal R
dc.contributor.authorWinkworth RC
dc.contributor.authorRamsfield TD
dc.contributor.editorMoricca S
dc.contributor.editorPanzavolta T
dc.date.accessioned2024-01-15T18:47:29Z
dc.date.accessioned2024-07-25T06:33:55Z
dc.date.available2021-03-19
dc.date.available2024-01-15T18:47:29Z
dc.date.available2024-07-25T06:33:55Z
dc.date.issued2021-03-19
dc.description.abstractA Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.
dc.description.confidentialfalse
dc.edition.editionMarch 2021
dc.identifier.author-urlhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000633686000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=c5bb3b2499afac691c2e3c1a83ef6fef
dc.identifier.citationMyrholm CL, Tomm BD, Heinzelmann R, Feau N, Hamelin RC, McDougal R, Winkworth RC, Ramsfield TD. (2021). Development of a rapid loop-mediated isothermal amplification assay for the detection of Dothistroma septosporum. Forests. 12. 3.
dc.identifier.doi10.3390/f12030362
dc.identifier.eissn1999-4907
dc.identifier.elements-typejournal-article
dc.identifier.numberARTN 362
dc.identifier.urihttps://mro.massey.ac.nz/handle/10179/70433
dc.languageEnglish
dc.publisherMDPI (Basel, Switzerland)
dc.publisher.urihttps://www.mdpi.com/1999-4907/12/3/362
dc.relation.isPartOfForests
dc.rights(c) 2021 The Author/s
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDNA-based diagnostics
dc.subjectLAMP
dc.subjectDothistroma needle blight
dc.titleDevelopment of a rapid loop-mediated isothermal amplification assay for the detection of Dothistroma septosporum
dc.typeJournal article
pubs.elements-id443070
pubs.organisational-groupOther
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