Non-polar lipid from greenshell mussel (Perna canaliculus) inhibits osteoclast differentiation
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Date
2021-12
Open Access Location
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Publisher
Elsevier Inc
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(c) The author/s
CC BY-NC-ND
Abstract
The osteoclast-dependent bone resorption process is a crucial part of the bone regulatory system. The excessive function of osteoclasts can cause diseases of bone, joint, and other tissues such as osteoporosis and osteoarthritis. Greenshell mussel oil (GSM), a good source of long chain omega-3 polyunsaturated fatty acids (LCn-3PUFAs), was fractionated into total lipid, polar lipid, and non-polar lipid components and their anti-osteoclastogenic activity tested in RAW 264.7 cell cultures. Osteoclast differentiation process was achieved after 5 days of incubation with RANKL in 24-well culture plates. Introducing the non-polar lipid fraction into the culture caused a lack of cell differentiation, and a reduction in tartrate-resistant acid phosphatase (TRAP) activity and TRAP cell numbers in a dose-dependent manner (50% reduction at the concentration of 20 μg/mL, p < 0.001). Moreover, actin ring formation was significantly diminished by non-polar lipids at 10-20 μg/mL. The bone digestive enzymes released by osteoclasts into the pit formation were also compromised by downregulating gene expression of cathepsin K, carbonic anhydrase II (CA II), matrix metalloproteinase 9 (MMP-9), and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1). This study revealed that the non-polar lipid fraction of GSM oil contains bioactive substances which possess potent anti-osteoclastogenic activity.
Description
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords
AA, Arachidonic acid, ALA, Alpha linolenic acid, CAII, Carbonic anhydrase II, DHA, Docosahexaenoic acid, DMSO, dimethyl sulfoxide, DPA, Docosapentaenoic acid, EPA, Eicosapentaenoic acid, FFAR, Free fatty acid receptor, GSM, Greenshell mussel, Greenshell mussel, LA, Linoleic acid, LPS, Lipopolysaccharide, MMP-9, Matrix metalloproteinase 9, MUFA, Monounsaturated fatty acid, NF-κB, Nuclear factor κB, NFATc1, Nuclear factor of activated T-cells, cytoplasmic 1, OA, Osteoarthritis, Omega 3 fatty acid, Osteoarthritis, Osteoclasts, Osteoporosis, PA, Palmitic acid, PPAR, Peroxisome proliferator activated receptor, PUFA, Polyunsaturated fatty acid, RANKL, Receptor activator of nuclear factor κB ligand, SFA, Saturated fatty acid, TRAP, Tartrate-resistant acid phosphatase
Citation
Siriarchavatana P, Kruger MC, Miller MR, Tian H, Wolber FM. (2021). Non-polar lipid from greenshell mussel (Perna canaliculus) inhibits osteoclast differentiation.. Bone Rep. 15. (pp. 101132-).