Non-polar lipid from greenshell mussel (Perna canaliculus) inhibits osteoclast differentiation

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dc.citation.volume15
dc.contributor.authorSiriarchavatana P
dc.contributor.authorKruger MC
dc.contributor.authorMiller MR
dc.contributor.authorTian HS
dc.contributor.authorWolber FM
dc.coverage.spatialUnited States
dc.date.accessioned2023-07-16T21:02:38Z
dc.date.accessioned2023-09-04T01:40:44Z
dc.date.available2021-09-24
dc.date.available2023-07-16T21:02:38Z
dc.date.available2023-09-04T01:40:44Z
dc.date.issued2021-12
dc.date.updated2023-07-12T22:24:46Z
dc.descriptionThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en
dc.description.abstractThe osteoclast-dependent bone resorption process is a crucial part of the bone regulatory system. The excessive function of osteoclasts can cause diseases of bone, joint, and other tissues such as osteoporosis and osteoarthritis. Greenshell mussel oil (GSM), a good source of long chain omega-3 polyunsaturated fatty acids (LCn-3PUFAs), was fractionated into total lipid, polar lipid, and non-polar lipid components and their anti-osteoclastogenic activity tested in RAW 264.7 cell cultures. Osteoclast differentiation process was achieved after 5 days of incubation with RANKL in 24-well culture plates. Introducing the non-polar lipid fraction into the culture caused a lack of cell differentiation, and a reduction in tartrate-resistant acid phosphatase (TRAP) activity and TRAP cell numbers in a dose-dependent manner (50% reduction at the concentration of 20 μg/mL, p < 0.001). Moreover, actin ring formation was significantly diminished by non-polar lipids at 10-20 μg/mL. The bone digestive enzymes released by osteoclasts into the pit formation were also compromised by downregulating gene expression of cathepsin K, carbonic anhydrase II (CA II), matrix metalloproteinase 9 (MMP-9), and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1). This study revealed that the non-polar lipid fraction of GSM oil contains bioactive substances which possess potent anti-osteoclastogenic activity.
dc.description.confidentialfalse
dc.edition.editionDecember 2021
dc.format.extent101132-
dc.identifier101132
dc.identifierS2352-1872(21)00389-2
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/34632003
dc.identifier.citationSiriarchavatana P, Kruger MC, Miller MR, Tian H, Wolber FM. (2021). Non-polar lipid from greenshell mussel (Perna canaliculus) inhibits osteoclast differentiation.. Bone Rep. 15. (pp. 101132-).
dc.identifier.doi10.1016/j.bonr.2021.101132
dc.identifier.eissn2352-1872
dc.identifier.elements-typejournal-article
dc.identifier.harvestedMassey_Dark
dc.identifier.issn2352-1872
dc.identifier.urihttp://hdl.handle.net/10179/19871
dc.languageeng
dc.publisherElsevier Inc
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S2352187221003892
dc.relation.isPartOfBone Rep
dc.rights(c) The author/s CC BY-NC-NDen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAA, Arachidonic acid
dc.subjectALA, Alpha linolenic acid
dc.subjectCAII, Carbonic anhydrase II
dc.subjectDHA, Docosahexaenoic acid
dc.subjectDMSO, dimethyl sulfoxide
dc.subjectDPA, Docosapentaenoic acid
dc.subjectEPA, Eicosapentaenoic acid
dc.subjectFFAR, Free fatty acid receptor
dc.subjectGSM, Greenshell mussel
dc.subjectGreenshell mussel
dc.subjectLA, Linoleic acid
dc.subjectLPS, Lipopolysaccharide
dc.subjectMMP-9, Matrix metalloproteinase 9
dc.subjectMUFA, Monounsaturated fatty acid
dc.subjectNF-κB, Nuclear factor κB
dc.subjectNFATc1, Nuclear factor of activated T-cells, cytoplasmic 1
dc.subjectOA, Osteoarthritis
dc.subjectOmega 3 fatty acid
dc.subjectOsteoarthritis
dc.subjectOsteoclasts
dc.subjectOsteoporosis
dc.subjectPA, Palmitic acid
dc.subjectPPAR, Peroxisome proliferator activated receptor
dc.subjectPUFA, Polyunsaturated fatty acid
dc.subjectRANKL, Receptor activator of nuclear factor κB ligand
dc.subjectSFA, Saturated fatty acid
dc.subjectTRAP, Tartrate-resistant acid phosphatase
dc.titleNon-polar lipid from greenshell mussel (Perna canaliculus) inhibits osteoclast differentiation
dc.typeJournal article
pubs.elements-id449056
pubs.organisational-groupOther
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