The role of pyruvate kinase in the regulation of glycolysis and gluconeogenesis in Propionibacterium shermanii : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University, New Zealand

dc.contributor.authorSmart, John Beresford
dc.date.accessioned2012-06-26T22:26:22Z
dc.date.available2012-06-26T22:26:22Z
dc.date.issued1980
dc.description.abstractPyruvate kinase catalyses the main irreversible reaction of glycolysis in Propionbacterium shermanii since the ATP-dependent phosphofructokinase is largely replaced by a pyrophosphate-dependent phosphofructokinase catalysing a reversible reaction. Measurement of activity of several glycolytic enzymes in glucose-, glycerol- and lactate-grown cells showed that pyruvate kinase activity is much higher than that of pyruvate, orthophosphate dikinase which catalyses the reversible interconversion of PEP and pyruvate. This poses problems in cells grown on lactate where gluconeogenesis must operate to supply hexoses and pentoses for biosynthesis. The regulatory properties of this enzyme were accordingly studied. The partially purified (140-fold) pyruvate kinase displayed sigmoidal kinetics for both of its substrates, phosphoenolpyruvate (PEP) and ADP. At pH 7.5 the interaction coefficient (nH) for PEP saturation in the absence of effectors was in the range 1.9-2.5 while for ADP saturation it was 1.7-2.1. The pyruvate kinase was shown to be activated by glucose 6-phosphate (G6P) at non-saturating (0.5 mM) PEP concentrations but other glycolytic and hexose monophosphate pathway intermediates and AMP were without effect. Half-maximal activation was obtained at 1 mM G6P. The presence of G6P decreased both the PEP0.5V and ADP0.5V values and the Hill interaction coefficient for each substrate. The enzyme was strongly inhibited by ATP and inorganic phosphate (Pi) at all PEP concentrations. At non-saturating (0.5 mM) PEP half-maximal inhibition was obtained at 1.8 mM ATP and 1.4 mM Pi. The inhibition of both Pi and ATP could be largely overcome by G6P. The G6P activation and other regulatory properties of the enzyme were pH dependent. On the basis of this in vitro study it was suggested that the activity of pyruvate kinase in vivo is determined by the balance between activators and inhibitors such that it is inhibited by ATP and Pi during gluconeogenesis while, during glycolysis, the inhibition is relieved by G6P. Such a mechanism requires that the G6P concentration should be sufficiently high when cultures are grown on glucose or glycerol but not on lactate. To verify this proposed mechanism the in vivo concentrations of a number of selected glycolytic intermediates were measured in P.shermannii growing under a range of nutritional conditions using batch and continuous cultures. The pyruvate kinase activator, G6P, was maintained at levels of 1-2 mM in lactate-grown cells but, when growing in the presence of high levels of glucose or glycerol, G6P was present at levels between 5 and 16 mM in the cell. F6P and FBP were always present at levels below 1.0 mM and 0.2 mM respectively in lactate- and glucose-grown cells but attained significantly higher levels on glycerol-grown cells. At high concentrations of glucose where high G6P levels were found, F6P was present at concentrations much lower than would be expected from the equilibrium constant of the phosphoglucoisomerase; this was not the case when high G6P levels were attained by growth on glycerol. The reason for this is not known. Levels of the inhibitor of pyruvate kinase activity, ATP, were maintained in the range 1-2 mM under all the conditions studied. At the in vivo levels of the substrates PEP and ADP this level of ATP would not by itself maintain pyruvate kinase in an inactive state in the presence of the 1-2 mM G6P shown to be present in lactate-grown cells. The other inhibitor, Pi, must also be important but the in vivo levels of this effector were not determined. A reinvestigation of the pyruvate kinase at the in vivo levels of substrates (PEP and ADP) and effectors (G6P and ATP) found in the cell indicated that, at levels above 10 mM Pi, concentrations of 5-15 mM G6P (as found in cells growing on glucose and glycerol) were required to activate the enzyme while concentrations of 1-2 mM G6P (as found in lactate-grown cells) were not able to overcome the inhibition by ATP and Pi. A concentration of 10 mM Pi in the cell does not seem unreasonable and at this concentration the proposed mechanism for control of the pyruvate kinase in vivo would be able to operate. During this investigation data were also collected on growth yields and carbon balances in both batch and continuous cultures during growth on the three substrates lactate, glucose and glycerol. Succinate was found to account for up to 26% of the products in glucose cultures but was only present at negligible levels in lactate cultures. Accumulation of succinate in the glucose cultures was highest in the later stages of growth of batch cultures and in carbonlimited continuous cultures when the G6P level was low. This suggests that regulation of pyruvate kinase by G6P may also serve to determine the relative flux via the PEP:carboxytransphosphorylase and pyruvate kinase enzymes - the carboxytransphosphorylase functioning to provide PPi for the PPi-dependent phosphofructokinase in glycolysis. It is also proposed that G6P may be involved in partitioning glucose metabolism via glycolysis and the hexose monophosphate pathway - high internal G6P concentrations favouring flow through the hexose monophosphate pathway and thus bypassing the PPi-dependent phosphofructokinase - however, this is highly speculative.en
dc.identifier.urihttp://hdl.handle.net/10179/3535
dc.language.isoenen
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectBacteriaen
dc.subjectPhysiologyen
dc.subjectPyruvatesen
dc.subjectMetabolismen
dc.subjectCarbohydratesen
dc.titleThe role of pyruvate kinase in the regulation of glycolysis and gluconeogenesis in Propionibacterium shermanii : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University, New Zealanden
dc.typeThesisen
massey.contributor.authorSmart, John Beresforden
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorMassey Universityen
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophy (Ph.D.)en
Files
Original bundle
Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
01_front.pdf
Size:
719.61 KB
Format:
Adobe Portable Document Format
Description:
Loading...
Thumbnail Image
Name:
02_whole.pdf
Size:
4.93 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
804 B
Format:
Item-specific license agreed upon to submission
Description: