Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol
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Date
2021-05-18
Open Access Location
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI (Basel, Switzerland)
Rights
(c) 2021 The Author/s
CC BY
CC BY
Abstract
A miniaturized quartz crystal microbalance (QCM) genosensor is proposed for sensitive and real-time detection of short ssDNA sequences (53 bp) or DNA extracted from Brettanomyces bruxellensis (Brett) yeast cells. The presence of Brett yeast causes a depreciation of the quality of aged fine wines, producing molecules of unpleasant odors and biogenic amines that are harmful to human health. More specifically, standard quartz crystal (S-QCM) and homemade 4 nm gold transmission electron microscopy (TEM)-grid patterned quartz (multi-TEM QCM) are herein proposed for biofunctionalization steps with different ssDNA sequences. By employing a rapid and efficient drop and collect protocol, the specific detection of 1 pg/µL ssDNA Brett of a short sequence and 100 ng/µL DNA of B. bruxellensis extracted from a wine sample (VR2008) is reported.
Description
Keywords
quartz crystal microbalance, multi-TEMs patterned crystal, genosensing, Brettanomyces bruxellensis, drop and collect protocol
Citation
Poggesi S, Zhou L, Bariani GC, Mittapalli R, Manzano M, Ionescu RE. (2021). Quartz crystal microbalance genosensing of brettanomyces bruxellensis yeast in wine using a rapid and efficient drop and collect protocol. Crystals. 11. 5.