Cortisol metabolism in the sheep (Romney breed) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science

Simple item page
dc.contributor.authorLangford, Alan Keith
dc.date.accessioned2014-01-29T20:13:04Z
dc.date.available2014-01-29T20:13:04Z
dc.date.issued1968
dc.description.abstractThe metabolism of Cortisol in the normal Romney ewe was investigated by analysis of the radioactive metabolites excreted in the urine following intravenous (I.V.) administration of 4-c14 cortisol. The metabolite glucuronic!os were hydrolysed with b-glucuronidase and extracted from the aqueous medium with ethyl acetate. The neutral fraction was divided into c-19 and c-21 metabolites by sequential elution from a florisil column. Extensive use was made of T.L.C. for the separation and analysis of each fraction before the quantitation of individual components. A series of experiments was performed with, surgically modified sheep involving collection of bile and urine both after I.V. injection of 4-c14 cortisol, and after intraduodenal infusion of radioactive biliary metabolites obtained from I.V. administration of 4-c14 corticol. The metabolites collected at each stage were analysed both qualitatively and quantitatively. The urine collected each hour for 18 consecutive days from a normal sheep, was subjected to colorimetric determination for a-ketol and 17-ketogenic steroid content. The data obtained was analysed for diurnal variation in chromogen output, and the daily secretion rate of cortisol was estimated.en
dc.identifier.urihttp://hdl.handle.net/10179/5049
dc.language.isoenen
dc.publisherMassey Universityen_US
dc.rightsThe Authoren_US
dc.subjectRomney sheepen
dc.subjectRomney Marsh sheepen
dc.subjectCortisolen
dc.titleCortisol metabolism in the sheep (Romney breed) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Scienceen
dc.typeThesisen
massey.contributor.authorLangford, Alan Keithen
thesis.degree.grantorMassey Universityen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Science (M.Sc.)en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
01_front.pdf
Size:
388.6 KB
Format:
Adobe Portable Document Format
Description:
Download
Thumbnail Image
Name:
02_whole.pdf
Size:
8.39 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
804 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses and Dissertations